| Optical module | |
| Excitation source | 96 LEDs (470 nm) |
| Emission filters | 520 / 550 nm (realplex2) |
| 520 / 550 / 580 / 605 nm (realplex4) | |
| Detector | 1 Photomultiplier (realplex2) |
| 2 Photomultiplier (realplex4) | |
| Dynamic | 9 orders of magnitudefrom starting copy number |
| Sensitivity | ≤50 fM fluorescein |
| Thermo module | |
| Sample capacity | 96 x 0.2 ml PCR tubes or one 96 PCR plate |
| (unskirted, semi-skirted, sikrted-as per SBS standard) | |
| Temperature control range of block | 4-99°C |
| Degree range of gradient, max. | 1-20°C (thermomodule Mastercycler ep) |
| 1-24°C (thermomodule Mastercycler ep S) | |
| Temperature control range of gradient | 30-99°C |
| Temperature of lid | 105°C |
| Block homogeneity | 35°C±0.3°C |
| 90°C±0.4°C | |
| Control accuracy | ±0.2°C |
| Heating speed * | approx.4°C/s (thermomodule Mastercycler ep) |
| approx.6°C/s (thermomodule Mastercycler ep S) | |
| Cooling speed * | approx.3°C/s (thermomodule Mastercycler ep) |
| approx.4.5°C/s (thermomodule Mastercycler ep S) | |
| Complete system | |
| Demensions /(W x D x H) | 26cm x 41cm x 39.6cm |
| Total weight | 24 kg |
| Weight of thermomodule | 17 kg |
| Weight of detection module | 7 kg |
| Voltage requirements | 200-240V, 50 Hz - 60 Hz |
| Power consumption | 800 W |
* Measured at block
Small, fast and flexible-the Mastercycler ep realplex real-time PCR system meets all the latest requirements of quantitative PCR applications. Extremely fast temperature ramping, short detection times and intuitive assay programming translate to huge time-savings so that more experiments can completed per day-always with accurate and reliable results. It′s flexibility is beyond compare-this completely open system allows you to use tubes, plates and reagents of your choice. No other real-time cycler on the market today offers such freedom.
Mastercycler ep realplex is compact in size, so it can fit in virtually any lab, no matter how limited the benchspace. It′s modular and therefore makes upgrading easy, for example: realplex allows you to upgrade your Mastercycler ep gradient S to a real-time PCR cycler at any time; the realplex2 module can be easily replaced by the realplex4 module if/when multiplexing becomes essential.
Innovative Eppendorf Thermal Sample Protection technology prevents premature heating of the samples, which significantly reduces the amplification of nonspecific PCR products during this heating phase. And a solid construction with minimal moving parts makes this a quiet, dependable unit that promotes consistently high sensitivity in your real-time PCR experiments.
This is a Licensed Real-Time Thermal Cycler(s) or Licensed Real-Time Temperature Cycling Instrument(s) under Applera's United States Patent No. 6,814,934 and corresponding claims in non-U.S. counterparts thereof, for use in research and for all other applied fields except human in vitro diagnostics. No right is conveyed expressly, by implication or by estoppel under any other patent claim.
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